By American Petroleum Institute
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Extra resources for API 21.1. Flow Measurement Using Electronic Metering Systems, Section 1 Electronic Gas Measurement
Its biochemical analysis was made possible by the development of techniques for the propagation of clonal populations of trypanosomes that undergo antigenic variation at a low rate and thus allow the production of large numbers of antigenically identical trypanosomes (Cross, 1975). The variant surface glycoprotein (VSG) is by far the most abundant component of the cell surface coat and it is composed of a single polypeptide species. A series of analyses of VSGs purified from a range of trypanosome clones all led to the conclusion that different VSGs are markedly different from each other in primary sequence.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), have consistently shown T. brucei to be contained within a monophyletic genus Trypanosoma. Due to limited numbers of taxa, however, none of these studies provided information on evolutionary relationships between T. brucei subspecies or within the subgenus Trypanozoon. More recently, a broad study of Trypanosoma spp. , 1999a) (Fig. 1) showed some support for a grouping of T. b. rhodesiense with T. b. brucei (66% bootstrap support), within a tight (100% bootstrap support) but undifferentiated clade of Trypanozoon taxa.
DNA sequence analysis Early rRNA sequence-based phylogenetic studies of T. , 1998) Trypanozoon taxa and thus provided little information about the evolution of T. brucei and subgenus Trypanozoon. , 1997), it is apparent that this under-representation and taxon imbalance severely affected the topology of the phylogenetic trees produced and frequently resulted in an incorrect placement of T. brucei and a paraphyletic representation of the genus Trypanosoma (see below). g. glyceraldehyde-3-phosphate dehydrogenase (GAPDH), have consistently shown T.
API 21.1. Flow Measurement Using Electronic Metering Systems, Section 1 Electronic Gas Measurement by American Petroleum Institute
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